GFP-CERTIFIED?FLUOFORTE?钙离子检测试剂盒

2024-01-09 17:43:14

Enzo Life Sciences的GFP-CERTIFIED??FLUOFORTE??Calcium assay kit提供了一种荧光分析方法,用于检测广泛生物靶标的细胞内钙动员情况。相对于其他商业化的染料,GFP-CERTIFIED??FLUOFORTE?染料是最亮和最灵敏的荧光钙指示剂。该试剂盒为贴壁和非贴壁细胞系提供了更少步骤,更低变异性,更简易的钙检测。此外,它既不需要洗涤步骤,也不需要添加外源性淬灭染料,避免了对受体-配体相互作用动力学的干扰。

Enzo Life Sciences/欣博盛生物的GFP-CERTIFIED??FLUOFORTE??Calcium assay kit可用于细胞内钙离子的高通量检测,钙离子是重要的第二信使,常用于初步筛选中监测G蛋白偶联受体激活。

产品特色

● 可监测表达GFP细胞中的钙离子动员情况

●?最明亮、最灵敏的荧光Ca2+指示剂

●?获得的 EC50和IC50值与使用钙离子荧光探针 Rhod-4 AM和Fluo-4 AM 相当

●?更大范围的检测窗口

●?使用方便,无需洗涤,无需淬灭染料

实验示例

GFP-CERTIFIED?FLUOFORTE?钙离子检测试剂盒——ENZO热销产品

GFP-CERTIFIED?FLUOFORTE?钙离子检测试剂盒——ENZO热销产品

图2. U-2 OS细胞分别与(A)GFP-CERTIFIED??FLUOFORTE?AM染料和(B)Rhod-2 AM染料孵育。洗涤细胞并用终浓度为100nM的ATP处理,并立即成像。GFP-CERTIFIED??FLUOFORTE??AM染料比Rhod-2 AM表现出更亮的荧光强度。

产品信息

产品货号

ENZ-51020-KP010/ ENZ-51020-KP100

产品名称

GFP-CERTIFIED??FLUOFORTE??Calcium assay kit / 欣博盛生物

规? ? ? ?格

10*96wells/100*96wells

短期保存

-20°C

长期保存

-80°C

试剂盒组分

Starter pack (Prod. No. ENZ-51020-KP010):

Reagent A (lyophilized GFP-CERTIFIED??FLUOFORTE??dye), 1 vial

Reagent B (dye efflux inhibitor), 10ml

Reagent C (Hanks’ buffer with 20mM HEPES, 100ml)

Sufficient for 10 x 96-well plates

High-throughput (Prod. No. ENZ-51020-KP100):

Reagent A (lyophilized GFP-CERTIFIED??FLUOFORTE??dye), 10 vials

Reagent B (dye efflux inhibitor), 10 x 10ml

Sufficient for 100 x 96-well plates

部分产品引用文献

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2.?horbol myristate acetate induces differentiation of THP-1 cells in a nitric oxide-dependent manner: Y.Y. Chang, et al.; Nitric Oxide 109, 33 (2021)

3.?Absolute proteomic quantification reveals design principles of sperm flagellar chemosensation: C. Tr?tschel, et al.; EMBO J. 39, e102723 (2020)

4.?Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium: J.N. Hansen, et al.; Elife 9, e57907 (2020)

5.?Interplay between sub-cellular alterations of calcium release and T-tubular defects in cardiac diseases: M. Scardigli, et al.; Front. Physiol. 9, 1474 (2018)

6.?Characterization of cholesterol homeostasis in sphingosine-1-phosphate lyase-deficient fibroblasts reveals a Niemann-Pick disease type C-like phenotype: H. Vienken, et al.; Sci. Rep. 7, 43575 (2017)

7.?Novel insights on the relationship between T-tubular defects and contractile dysfunction in a mouse model of hypertrophic cardiomyopathy: C. Crocini, et al.; J. Mol. Cell. Cardiol. 91, 42 (2016)

8.?Sulfur dioxide protects against collagen accumulation in pulmonary artery in association with downregulation of the transforming growth factor β1/smad pathway in pulmonary hypertensive rats: W. Yu, et al.; J. Am. Heart Assoc. 5, e003910 (2016)

9.?T-tubular electrical defects contribute to blunted β-adrenergic response in heart failure: C. Crocini, et al.; Int. J. Mol. Sci. 17, 1471 (2016)

10.?Defects in T-tubular electrical activity underlie local alterations of calcium release in heart failure: C. Crocini, et al.; PNAS 111, 15196 (2014)

文章来源:https://blog.csdn.net/Neobioscience/article/details/135485119
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