ROS-ID?总活性氧/超氧化物检测试剂盒

Enzo Life Sciences的ROS-ID??Total ROS/Superoxide detection kit包括两种荧光染料作为主要成分：非荧光、可渗透细胞的氧化应激检测试剂（绿色，Ex/Em 490/525 nm）与多种活性物质（过氧化氢、过氧亚硝酸根和羟基）直接反应，产生绿色荧光产物。超氧化物检测试剂（橙色，Ex/Em 550/620 nm）是一种可渗透细胞的探针，可与超氧化物特异性反应，产生橙色荧光产物。试剂盒还提供Pycyanin（ROS诱导剂）和NAC（ROS抑制剂）。

产品特点

●?用于检测活细胞中超氧化物的产生

●?与细胞培养基的主要成分（酚红，FBS和BSA）兼容

●?已通过显微镜、流式细胞仪和微孔板等应用验证

实验示例

图1. 在加入了ROS/超氧化物检测试剂并用绿脓菌素处理的 HeLa 细胞中，通过荧光显微镜对活性氧的形成进行了分析。在绿色通道中监测一般氧化应激水平，而在橙色通道中检测超氧化物的产生。用 NAC（一种通用的 ROS 抑制剂）预处理可防止 ROS 的形成。

图2. 使用ROS-ID??Total ROS/Superoxide detection kit和ROS清除剂/抑制剂分析用抗霉素A（AMA，特异性超氧化物诱导剂）、叔丁基-过氧化物（TBHP，特异性过氧化物诱导剂）和绿脓菌素（通用ROS诱导剂）处理的HeLa细胞中ROS的产生。使用U-2 OS和CHO K1细胞获得了类似的结果。

图3. 通过流式细胞术分析Hela细胞中ROS的形成。数据以绿脓菌素（ROS/SO诱导剂）、TBHP（ROS 诱导剂）和 AMA（超氧化物诱导剂）处理后的阳性百分比表示。绿色柱表示ROS 阳性率%，橙色柱表示超氧化物阳性率%。

产品信息

产品货号	ENZ-51010 / 欣博盛生物
产品名称	ROS-ID??Total ROS/Superoxide detection kit
别名	Reactive oxygen species / Superoxide
规格	1*1Kit (200 fluorescence microscopy assays or 50 flow cytometry assays or 2x96 microplate assays)
使用/稳定性	With proper storage, the kit components are stable up to the date noted on the product label.? Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.
短期保存	-20°C
长期保存	-80°C
试剂盒组分	Oxidative Stress Detection Reagent (Green), 300 nmoles Superoxide Detection Reagent (Orange), 300 nmoles ROS Inducer (Pyocyanin), 1 μmole ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg Wash Buffer Salts, 1 pack
应用	Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS

部分产品引用文献

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